11/11/2023 0 Comments Dot blot kit![]() Phospho-IRF-3 (Ser386) (E7J8G) XP Rabbit mAb recognizes endogenous levels of IRF-3 protein only when phosphorylated at Ser386. Enhancer™ Membranes: Precut nitrocellulose or nylon membranes. Each antibody in the Type I Interferon Induction and Signaling Antibody Sampler Kit recognizes endogenous levels of its target protein.Enhancer™ Sample Tubes: 8 well strips for sample application.The supernatant from each tube was collected for measurement of protein content using Pierce BCA protein assay kit from Thermo Fisher and for Western blot analysis. In fact, both enzyme-linked immunosorbent assay. Protein Binding Buffer: For increased protein binding efficiency. As an alternative, Dot blot analysis was developed to simplify the process of Western blot analysis.Production of dot blots for 1-384 samples.O Nucleic acid samples with specific hybridization probe. O Multiple protein samples with specific antibodies. No expensive electronic equipment required.Apply up to 384 protein or nucleic acid samples.Increase dot blot sensitivity by >10 fold.In less than 30 minutes you can prepare a dot blot or an array of up to 384 samples. No risk of cross contamination or waste of samples. Load 2-5µl samples into the sample application tubes which allow deposition of the samples on the binding membranes by a point of entry capillary action. The Exosomal Marker Antibody Sampler Kit provides an economical means to evaluate the presence of exosomal markers. A total of 384 samples may be applied where spots or samples are 3-4mm apart. The application tray may be flipped four times to apply 4 x 96 samples on a 12 x 9cm membrane. Enhancer™ is provided with a flip-application tray which accommodates 96 application tubes. Grid formation allows for easy identification of samples after probing.Įach Enhancer™ is provided with a set of 12 x 8 well application strips. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion.Įnhancer™ prevents sample waste and cross contamination of samples. Enhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |